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Comparison of the N‐Linked Oligosaccharide Structures of the Two Major Human Myelin Glycoproteins MAG and P 0 : Assessment and Relative Occurrence of Oligosaccharide Structures by Serial Lectin Affinity Chromatography of 14 C‐Glycopeptides
Author(s) -
Burger Danielle,
Perruisseau Geneviève,
Simon Maryline,
Steck Andreas J.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb09334.x
Subject(s) - oligosaccharide , fucose , glycoprotein , glycopeptide , chemistry , residue (chemistry) , lectin , biochemistry , carbohydrate conformation , n acetylglucosamine , acetylation , trisaccharide , stereochemistry , polysaccharide , enzyme , gene , antibiotics
The N‐linked Oligosaccharide structures of human myelin‐associated glycoprotein (MAG) and P 0 have been characterized by serial lectin affinity chromatography (SLAC) of 14 C‐glycopeptides. 14 C‐Glycopeptides were prepared from purified MAG derivative and P 0 by extensive proteolytic digestion and N ‐ 14 C‐acetylation. Assuming that all the 14 C‐glycopeptides were radiolabelled to the same specific radioactivity, the relative occurrence of the Oligosaccharide structures was correlated to the amount of incorporated radioactivity. Sixteen and 15 fractions were generated by SLAC of MAG and P 0 14 C‐glycopeptides, respectively. Despite this tremendous structural heterogeneity, the Oligosaccharide “fingerprints’ of MAG and P 0 obtained by SLAC displayed similarities: (a) of the three types of N‐linked oligosaccharides, the complex type accounted for 80.4% and 94.9% of MAG and P 0 radioactivity, respectively; (b) biantennary complex oligosaccharides were the major structures present on MAG and P 0 ; (c)60% of MAG and P 0 oligosaccharides possessed a bisecting N ‐acetylglucosamine residue; and (d) large amounts of oligosaccharides with an α(l‐6)fucose residue were found in both MAG and P 0 and, noticeably, 25% of the tri‐ and/or tetraantennary and 90% of the bisected biantennary oligosaccharides of both glycoproteins contained α(l‐6)fucose residues in the core. This study demonstrates that MAG and P 0 , both belonging to the immunoglobulin superfamily, display structural similarities in their N‐linked Oligosaccharide contents.