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Time‐Dependent Inhibition of Stimulatéd Adrenal Catecholamine Release by Staurosporine
Author(s) -
Maurer Jennifer A.,
McKay Dennis B.
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb08479.x
Subject(s) - staurosporine , protein kinase c , chromaffin cell , protein kinase a , catecholamine , kinase , secretion , adrenal medulla , endocrinology , medicine , biology , chemistry , microbiology and biotechnology
Staurosporine, a potent inhibitor of protein kinases, is used to study the involvement of protein kinases in cellular processes. In the present studies, the effect of prolonged staurosporine treatment on catecholamine secretion in cultured bovine adrenal chromaffin cells was examined. Staurosporine inhibits catecholamine release stimulated by 10 μ M nicotine, depolarizing concentrations of potassium (56 m M KCl), and 2 m M BaCl 2 . The effects of staurosporine on KCl‐stimulated release are time dependent, with a half‐time of ∼50 min and a maximal inhibition at 2 h. Our results indicate that activation of a staurosporine‐sensitive protein kinase is not directly involved in the stimulus‐secretion coupling process. This does not rule out the possibility that Ca 2+ /phospholipid‐dependent protein kinase or other protein kinases may acutely modulate release. However, these results suggest that a protein(s), which is phosphorylated by a staurosporine‐sensitive protein kinase(s), is required to maintain the integrity of the stimulus‐secretion coupling process.