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Simultaneous Effects of p ‐Chloroamphetamine, d ‐Fenfluramine, and Reserpine on Free and Stored 5‐Hydroxytryptamine in Brain and Blood
Author(s) -
Martín Francisco,
Artigas Francesc
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb08356.x
Subject(s) - fenfluramine , reserpine , extracellular , p chloroamphetamine , endocrinology , medicine , serotonin , brain tissue , 5 hydroxyindoleacetic acid , intracellular , chemistry , extracellular fluid , serotonergic , biochemistry , receptor
The effects of acute treatment with p ‐chloroamphetamine, d ‐fenfluramine, and reserpine on intracellular (brain tissue and whole blood) and extracellular (CSF and platelet‐free plasma) compartments of 5‐hydroxytryptamine (5‐HT) in the brain and blood of the same rats have been examined. These treatments affected 5‐HT in brain tissue and whole blood similarly ( r = 0.823). Reserpine significantly reduced both intracellular pools at 2 and 24 h. p ‐Chloroamphetamine and d ‐fenfluramine were more effective on brain tissue 5‐HT. The concentration of 5‐HT in CSF was significantly increased by all treatments. p ‐Chloroamphetamine induced a dramatic 70‐fold increase of CSF 5‐HT, paralleling a 42% decrease in brain tissue. d ‐Fenfluramine significantly increased CSF 5‐HT to 212% of controls and reduced whole brain 5‐HT (‐23%). The effects of p ‐chloroamphetamine and d ‐fenfluramine on 5‐HIAA in brain, CSF, and plasma were nonsignificant. Individual values of 5‐hydroxyindoleacetic acid (5‐HIAA) in CSF and brain were highly correlated ( r = 0.855), indicating that CSF 5‐HIAA reflects well the concentration of 5‐HIAA in brain tissue. Yet the intra‐ and extracellular concentrations of 5‐HIAA were unrelated to the 5‐HT changes. This indicates that CSF 5‐HIAA does not reflect the active (extracellular) compartment of 5‐HT in brain.