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Activation by Nitric Oxide of Guanylate Cyclase in Endothelial Cells from Brain Capillaries
Author(s) -
Marsault Robert,
Frelin Christian
Publication year - 1992
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1992.tb08334.x
Subject(s) - sodium nitroprusside , bradykinin , neurotensin , nitric oxide , chemistry , endothelial stem cell , medicine , endocrinology , neuroblastoma , receptor , neuropeptide , biology , biochemistry , cell culture , in vitro , genetics
Abstract: Endothelial cells (ECs) from brain microvessels respond to exogenous nitric oxide (NO) donor molecules ( N ‐ethoxycarbonyl‐3‐morpholinosydnonimine and sodium nitroprusside) with large (> 15‐fold) increases in cyclic GMP (cGMP) levels. Comparable actions of sodium nitroprusside were observed in vascular smooth muscle cells and in neuroblastoma cells. Coculturing brain capillary ECs in the presence of N1E‐115 neuroblastoma cells increased their cGMP levels fourfold. A further increase was observed in the presence of 50 n M neurotensin, although brain capillary ECs lack receptor sites for neurotensin. The neuroblastoma cell‐dependent formation of cGMP was suppressed by 0.1 m M l‐ N G ‐monomethylarginine, indicating that NO, produced by N1E‐115 cells in response to neurotensin, activated guanylate cyclase in brain capillary ECs. Similarly, culturing brain capillary ECs in the presence of aortic ECs increased their cGMP content in a manner that was amplified by bradykinin and that was inhibited by l‐ N G ‐monomethylarginine. Bradykinin had no action in pure cultures of brain capillary ECs. It is concluded that brain capillary ECs express high levels of guanylate cyclase activity that could be activated by exogenous NO donor molecules and by NO produced by neuroblastoma cells and by aortic ECs in response to specific agonists. Brain capillary ECs are thus potential target cells for brain‐derived NO.