Premium
Characterization of the Carbohydrate Chain on the Substance P Receptor in the Rat Brain Cortex: Effect of Lectins on [ 3 H]Substance P Binding
Author(s) -
Takamatsu Hiroyuki,
Tani Yoshie,
Akiyama Mitoshi,
Nakata Yoshihiro,
Segawa Tomio
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb11447.x
Subject(s) - carbohydrate , chemistry , substance p , receptor , biochemistry , cortex (anatomy) , stereochemistry , biology , neuroscience , neuropeptide
The characteristics of the carbohydrate chain on the rat cerebral cortical substance P (SP) receptor were studied. We examined the effects of pretreatment with three lectins (concanavalin A, wheat germ agglutinin, lens culinaris agglutinin) on the [ 3 H]SP binding activities. Each lectin can bind to the specific carbohydrate chain. Among these lectins, only concanavalin A inhibited specific [ 3 H]SP binding by reducing the affinity of the binding sites. The inhibitory action of concanavalin A was dose‐dependent and diminished by the addition of α‐methyl‐D‐mannoside. The present results suggest that the rat cortical SP receptor has either a biantennary complex‐type or a high mannose‐type of carbohydrate chain, and that the carbohydrate chain is implicated in the SP binding activity of the SP receptor system.