Premium
Incubation of Tissue Slices in the Absence of Ca 2+ and Mg 2+ Can Cause Nonspecific Damage
Author(s) -
Lonart Gyorgy,
Zigmond Michael J.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb11445.x
Subject(s) - incubation , biophysics , chemistry , neuroscience , pathology , biology , biochemistry , medicine
Supervision of striatal slices with a medium deficient in Ca 2+ and Mg 2+ caused a large and sustained increase in release of lactate dehydrogenase, a finding indicative of the disruption of plasma membranes. This was associated with an efflux of dopamine (DA) and the depletion of DA from the tissue. In addition, whereas DA efflux was stimulated by either D‐amphetamine (10 μ M ) or L‐glutamate (10 m M ) in the absence of Ca 2+ , these effects were greatly reduced when Mg 2+ also was withdrawn from the buffer. These results suggest that (a) incubation in a Ca 2+ /Mg 2+ ‐free buffer disrupts plasma membranes, (b) this disruption affects dopaminergic neurons as well as those of other striatal elements, and (c) the failure of a treatment to stimulate DA release in a Ca 2+ /Mg 2+ ‐free buffer cannot be used as a test of Ca 2+ dependence.