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Are Several G Proteins Involved in the Different Effects of Endothelin‐1 in Mouse Striatal Astrocytes?
Author(s) -
Marin P.,
Delumeau J. C.,
DurieuTrautmann O.,
Nguyen D.,
Prémont J.,
Strosberg A. D.,
Couraud P. O.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb11421.x
Subject(s) - pertussis toxin , stimulation , inositol phosphate , g protein , biology , bordetella pertussis , intracellular , receptor , microbiology and biotechnology , inositol , biochemistry , chemistry , endocrinology , genetics , bacteria
High‐affinity specific receptors of endothelin (ET‐1) were identified on primary cultures of mouse embryo striatal astrocytes by binding experiments performed with 125 I‐ET‐1. Stimulation of production of inositol phosphates, a biphasic increase of the intracellular calcium concentration, and inhibition of cyclic AMP accumulation were observed in the same cells under ET‐1 stimulation. Pretreatment of these cells with Bordetella pertussis toxin affected these effects to different extends, an observation suggesting that they are mediated by multiple transduction pathways, possibly involving several guanine nucleotide‐binding proteins.