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Identification of B 2 Bradykinin Binding Sites on Cultured Cortical Astrocytes
Author(s) -
Cholewinski A. J.,
Stevens G.,
McDermott A. M.,
Wilkin G. P.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb08314.x
Subject(s) - bradykinin , receptor , astrocyte , binding site , biology , bradykinin receptor , microbiology and biotechnology , chemistry , medicine , endocrinology , biochemistry , central nervous system
Bradykinin was found to bind to specific high‐affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B 2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single K D of 16.6 ± 2.6 n M and a B max of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B 2 subtype.