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Ca 2+ ‐Independent Release of Glutamate During In Vitro Anoxia in Isolated Nerve Terminals
Author(s) -
Rubio Inmaculada,
Torres Magdalena,
MirasPortugal María Teresa,
SánchezPrieto Jose
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb08274.x
Subject(s) - glutamate receptor , in vitro , glutamic acid , anaerobic exercise , biochemistry , chemistry , anaerobic glycolysis , efflux , biology , biophysics , glycolysis , metabolism , amino acid , receptor , physiology
The effects of in vitro anoxia on the release of glutamate in isolated nerve terminals were studied. The extrasynaptosomal concentration of glutamate ([Glu] cxt ) under aerobic conditions was 2.3 μ M and increased to 4.9 μ M after 10 min of anoxia. However, when synaptosomes were incubated in the presence of lactate plus pyruvate instead of glucose, to prevent anaerobic glycolysis, anoxia induced an eightfold increase in the [Glu] cxt The accumulation of glutamate in the external medium during anoxia was Ca 2+ in dependent and insensitive to a significant reduction of the Ca 2+ ‐dependent release of the amino acid. These results indicate that a Ca 2+ ‐independent efflux of cytoplasmic glutamate occurs during in vitro anoxia in isolated nerve terminals.