Premium
Kininogen and Kinin in Experimental Spinal Cord Injury
Author(s) -
Xu Jian,
Hsu Chung Y.,
Junker Herman,
Chao Steven,
Hogan Edward L.,
Chao Julie
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb08246.x
Subject(s) - kininogen , kinin , spinal cord , spinal cord injury , bradykinin , high molecular weight kininogen , evans blue , medicine , chemistry , endocrinology , receptor , psychiatry
Activation of the kallikrein‐kinin system has been implicated in the pathogenesis of vasogenic brain edema and posttraumatic vascular injury. We determined the levels of kininogen and kinin in an experimental spinal cord injury model in the rat. Kininogen content in traumatized cord segments increased in a time‐dependent manner. Western blot analysis showed that the kininogen in traumatized cord comigrates with 68K low‐molecular‐weight kininogen or T‐ kininogen. Trypsin treatment of the kininogen in traumatized cord released both bradykinin and T‐kinin, which were separated by HPLC and quantified with a kinin radioimmu‐ noassay. Endogenous kinin levels in the frozen spinal cord also increased up to 40‐fold 2 h after injury as compared with controls. The results demonstrate an increased accumulation of kininogen and its conversion to vasoactive kinins in experimental spinal cord injury.