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Redox Modulation of N ‐Methyl‐D‐Aspartate‐Stimulated Neurotransmitter Release from Rat Brain Slices
Author(s) -
Woodward John J.,
Blair Robert
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb06422.x
Subject(s) - nmda receptor , dithiothreitol , chemistry , antagonist , stimulation , glutamate receptor , endogeny , biophysics , medicine , endocrinology , biochemistry , receptor , biology , enzyme
Rat brain cortical slices released tritiated norepi‐nephrine ([ 3 H]NA) during a 2‐min stimulation with N ‐methyl‐ d ‐aspartate (NMDA). Dithiothreitol (DTT; 0.1–5 m M ), present for 6 min prior to stimulation, dose‐depen‐dently increased the release of [ 3 H]NA from cortical slices stimulated with a maximally effective concentration of NMDA (500 μM ). Similar results were observed for [ 3 H]NA release from hippocampal slices and tritiated and endogenous dopamine release from striatal slices. DTT treatment also markedly shifted the dose‐response curve of NMDA to the left. Cortical slices released approximately the same amount of [ J H]NA with 10 μM NMDA following DTT treatment (about 5%) as non‐DTT‐treated control slices did with 500 μ M NMDA. The effects of DTT were fully reversed by subsequent treatment with 5,5′‐dithio‐bis(2‐nitrobenzoic acid) (DTNB; 0.5 m M ). DTT treatment did not significantly alter the ability of magnesium (1.3 m M ) or the polyamine antagonist arcaine to block the NMDA‐stimulated release of [ 3 H]NA. In contrast, DTT treatment significantly attenuated the antagonist effects of the competitive glycine antagonist, 7‐chlorokynurenic acid, and the competitive NMDA antagonist, 2‐aminophosphonopentanoic acid. These results suggest that oxidation and reduction of disulfide bonds located within the NMDA receptor complex might regulate the activation of the NMDA receptor. This could have important consequences in vivo if endogenous oxidizing/reducing systems are found to have similar effects on NMDA‐stimulated responses.