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Presence of Various Carbohydrate Moieties Including β‐Galactose and N ‐Acetylglucosamine Residues on Solubilized Porcine Brain Neurokinin‐1/Substance P Receptors
Author(s) -
Liu Ya Fang,
Quirion Remi
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb06408.x
Subject(s) - biochemistry , concanavalin a , receptor , lectin , wheat germ agglutinin , galactose , fucose , biology , n acetylglucosamine , mannose , substance p , ricinus , chemistry , neuropeptide , in vitro , enzyme
Abstract Neurokinin‐1 (NK‐1)/substance P (SP) receptors were solubilized using 10 m M 3‐[(cholamidopropyl)‐dimethylammonio]‐1‐propanesulfate from porcine striatal membranes (solubilization yield, 80%). In solubilized preparations, [ 3 H]SP apparently bound to a single class of high‐affinity sites ( K D = 0.82 ± 0.13 n M ) as in membrane ho‐mogenates. The ligand selectivity pattern observed in both membrane and solubilized receptor preparations indicated that [Sar 9 , Met(O 2 ) 11 ]SP = SP ± senktide = [Nle 10 ]neurokinin A 4–10 .This suggests the selective labeling of the NK‐1 receptor class in both assays. Solubilized receptors were retained on agarose‐coupled lectins that bind N ‐acetylglucosamine‐galactose and β‐galactose (Ricinus communis I and Ricinus communis II), mannose (concanavalin A and lentil), and N ‐ acetylglucosamine (wheat germ agglutinin) but not on lectins binding fucose ( Lotus A) and N ‐acetylgalactosamine ( Dolichos biflorus A). Thus, it appears that porcine brain NK‐1/SP receptors are enriched with various carbohydrate moieties, β‐galactose and N ‐acetylglucosamine‐galactose residues being especially abundant. This situation is rather different from that in various other members of the rhodopsin seven‐trans‐membrane receptor superfamily.