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D 1 Dopamine Receptors of NS20Y Neuroblastoma Cells Are Functionally Similar to Rat Striatal D 1 Receptors
Author(s) -
Lovenberg Timothy W.,
Roth Robert H.,
Nichols David E.,
Mailman Richard B.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb06352.x
Subject(s) - receptor , dopamine receptor , dopamine , neuroblastoma , neuroscience , chemistry , endocrinology , medicine , biology , microbiology and biotechnology , biochemistry , cell culture , genetics
: Dopamine or agonists with D 1 receptor potency stimulated cyclic AMP (cAMP) accumulation in whole cell preparations of NS20Y neuroblastoma cells. The accumulation of cAMP after D 1 stimulation was rapid and linear for 3 min. Both dopamine and the novel D 1 receptor agonist dihydrexidine stimulated cAMP accumulation two‐to threefold over baseline. The pseudo‐ K m for dopamine was ⋍2 μ M , whereas for dihydrexidine it was ⋍30 n M. The effects of both drugs were blocked by either the D 1 ‐selective antagonist SCH23390 ( K i , 0.3 n M ) or the nonselective antagonist (+)‐butaclamol ( K i , 5 n M ). Both (−)‐butaclamol and the D 2 ‐selective antagonist (−)‐sulpiride were ineffective ( K i > 3 μ M ). Forskolin (10 μ M ), prostaglandin E 1 (1 μ M ), and adenosine (10 μ M ) also stimulated cAMP accumulation, but none were antagonized by SCH23390 (1 μ M ). Finally, muscarinic receptor stimulation (100 μ M carbachol) inhibited both D 1 ‐and forskolin‐stimulated increases in cAMP accumulation by 80%. The present results indicate that NS20Y neuroblastoma cells have D 1 receptors that are coupled to adenylate cyclase, and that these receptors have a pharmacological profile similar to that of the D 1 receptor(s) found in rat striatum.