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A Specific Trarisduction Mechanism for the Glutamate Action on Phosphoinositide Metabolism via the Quisqualate Metabotropic Receptor in Rat Brain Synaptoneurosomes: I. External Na + Requirement
Author(s) -
Guiramand Janique,
Vignes Michel,
Mayat Ebrahim,
Lebrun Fabienne,
Sassetti Isabella,
Récasens Max
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb06343.x
Subject(s) - depolarization , veratridine , tetrodotoxin , metabotropic receptor , biophysics , chemistry , glutamate receptor , mechanism of action , biochemistry , biology , receptor , sodium channel , sodium , organic chemistry , in vitro
: The characteristics of the transduction mechanism(s) activated by glutamate (Glu) via the quisqualate metabotropic receptor, as well as by depolarizing agents, to trigger formation of inositol phosphates (IPs) were investigated in 8‐day‐old rat forebrain Synaptoneurosomes. The replacement of external Na + by various compounds (Li + , Tris + , N ‐methyl‐D‐glucamine + , and sucrose) induces an increase in basal accumulation of IPs and depolarizes synaptoneurosome membranes. Under these conditions, Gluand K + ‐induced accumulations of IPs are inhibited, whereas the carbachol (Carb)‐elicited response of IPs parallels the basal one. Agents increasing Na + influx, such as veratridine and monensin, depolarize Synaptoneurosomes and stimulate formation of IPs. These stimulations are not additive with responses of IPs elicited by Glu or K + . These data suggest that (a) Glu activates phosphoinositide metabolism via a specific mechanism (distinct from that of cholinergic agonists), (b) depolarizing agents and Glu share at least one common intermediate step in their mechanisms of activation of the metabolism of IPs, and (c) the depolarization may correspond to this common step. ID addition, Na + seems to be required for Glu stimulation of metabolism of IPs. The depolarization associated with the action of Glu on formation of IPs results neither from an influx via tetrodotoxin‐sensitive voltage‐dependent Na+ channels nor from an entry via the classically characterized Na + /Ca 2+ or Na + /H + exchangers. In fact, tetrodotoxin (2 μ M ) has no effect on the Glu‐or K + ‐elicited response of IPs. Amiloride (>50 μ M ) and some of its derivatives similarly inhibit not only Glu‐and K + ‐but also Carb‐evoked formation of IPs.