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Inhibition of Glutamate Release by Arachidonic Acid in Rat Cerebrocortical Synaptosomes
Author(s) -
Herrero Inmaculada,
MirasPortugal Maria Teresa,
SańchezPrieto Jose
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb03805.x
Subject(s) - arachidonic acid , glutamate receptor , depolarization , synaptosome , tetraethylammonium , inhibitory postsynaptic potential , chemistry , glutamic acid , biochemistry , biology , biophysics , potassium , endocrinology , in vitro , amino acid , receptor , enzyme , organic chemistry
The action of arachidonic acid on glutamate release in rat cerebrocortical synaptosomes was investigated. The Ca 2+ ‐dependent release of glutamate evoked by 4‐aminopyridine (4‐AP) was inhibited by arachidonic acid (0.5–10 μM ), but the KC1‐evoked release was not modified. The Ca 2+ ‐independent release of glutamate was insensitive to low concentrations of arachidonic acid, but higher concentrations of this free fatty acid (30 μM ) induced a slow efflux of cytoplasmic glutamate. The decrease in the Ca 2+ ‐dependent release of glutamate by arachidonic acid was consistent with a reduction in both the depolarization and the subsequent rise in the cytoplasmic free Ca 2+ concentration induced by 4‐AP in the nerve terminal. The inhibitory action by arachidonic acid observed in glutamate release was reversed in the presence of the K + ‐channel Mocker tetraethyl‐ammonium.