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Intragenic Sequences Affect the Expression of the Gene Encoding Glial Fibrillary Acidic Protein
Author(s) -
Sarkar Srijata,
Cowan Nicholas J.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb03799.x
Subject(s) - gene , glial fibrillary acidic protein , biology , gene expression , heterologous expression , microbiology and biotechnology , regulatory sequence , heterologous , regulation of gene expression , gfap stain , genetics , recombinant dna , immunohistochemistry , immunology
We show that the expression of the gene encoding glial fibrillary acidic protein (GFAP) gene is affected by at least three as‐acting elements. A positive regulatory element that is located between nucleotides ‐1, 631 and ‐1, 479 can confer cell type‐specific expression on a heterologous gene. A second regulatory element is located between nucleotides ‐97 and ‐80. The third is a negative regulatory element that is located within the first intron of the gene. Deletion of this element activates GFAP expression in HeLa cells, and affects promoter function in glioma cells.