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Purification and Properties of p 103, a Novel 103‐kDa Component of Postsynaptic Densities
Author(s) -
Hayes Nandini V. L.,
Rayner Denise A.,
Baines Anthony J.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb03766.x
Subject(s) - postsynaptic potential , spectrin , cytoskeleton , cell fractionation , isoelectric focusing , postsynaptic density , actin , size exclusion chromatography , biology , biophysics , biochemistry , membrane , chemistry , microbiology and biotechnology , enzyme , receptor , cell
A 103‐kDa protein present in membrane cytoskeletal preparations from bovine brain has been identified. We have purified this protein to >95% homogeneity using gel filtration and ion‐exchange chromatography. This protein, p 103, is an asymmetric dimer in dilute solution and has two major variants that can be distinguished by isoelectric focussing, pI 5.60 and 5.75. Using subcellular fractionation, it is most enriched in postsynaptic densities. Immunolocalization with anti‐p 103‐specific antibodies reveals that it is confined to the dendrites and perikarya; it is apparently absent from spinal cord axons. It coextracts from brain membrane‐skeletal preparations with brain spectrin and actin, but in vitro, it does not interact with them.