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Dopamine D 2 Receptors Attenuate Phosphatidylisitol 4,5‐Bisphosphate in Synaptosomal Membranes from Rat Neostriatum
Author(s) -
Eule Gabriel,
Euler Anne
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb02572.x
Subject(s) - dopamine , dopamine receptor , chemistry , receptor , neuroscience , synaptic membrane , biology , biochemistry
The effects of dopamine on [ 32 P]ATP‐labelled phosphatidylinositol 4‐phosphate, phosphatidylinositol 4,5‐bisphosphate, and phosphatidic acid were analyzed by TLC in synaptosomal membranes of the rat neostriatum. The incorporation of 32 P into these compounds was found to be stable within 1 min and was maintained during the 30 min of incubation. Dopamine (0.1–10μ M ) was found to attenuate the levels of phosphatidylinositol 4,5‐bisphosphate without affecting the levels of phosphatidylinositol 4‐phosphate or phosphatidic acid. The maximal decrease (−35 ± 4%) was reached at 10 μ M of dopamine after 30 min of incubation. The dopamine (0.1 μ M )‐induced decrease was blocked by the D 2 selective antagonist raclopride (1 μ M ), but not by the D 1 selective antagonist SCH 23390 (1 μ M ). These findings indicate the existence of an intramembrane coupling of dopamine D 2 receptors to phosphoinositide turnover and may underlie some of the physiological effects of D 2 receptor stimulation.