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Immunocytochemical Demonstration of Na + ,K + ‐ATPase in Internodal Axolemma of Myelinated Fibers of Rat Sciatic and Optic Nerves
Author(s) -
Mata Marina,
Fink David J.,
Ernst Stephen A.,
Siegel George J.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb02114.x
Subject(s) - axolemma , immunocytochemistry , antiserum , polyclonal antibodies , electron microscope , sciatic nerve , ultrastructure , atpase , chemistry , biophysics , anatomy , biology , central nervous system , biochemistry , myelin , neuroscience , enzyme , endocrinology , physics , optics , antibody , immunology
We used postembedding electron microscopic immunocytochemistry with colloidal gold to determine the ultrastructural distribution of Na + ,K + ‐ATPase in the sciatic and optic nerves of the rat. Using a polyclonal antiserum raised against the denatured catalytic subunit of brain Na + ,K + ‐ATPase, we found immunoreactivity along the internodal axolemma of myelinated fibers in both nerves. This antiserum did not produce labeling of nodal axolemma. These results suggest that an important site of energy‐dependent sodium‐potassium exchange is along the internodal axolemma of myelinated fibers in the mammalian CNS and PNS and that there may be differences between the internodal and nodal forms of the enzyme.