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Biosynthesis of Prostaglandins D 2 and E 2 in Chick Dorsal Root Ganglion During Development
Author(s) -
Vesin MarieFrançoise,
Droz Bernard
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb02111.x
Subject(s) - arachidonic acid , biosynthesis , enzyme , dorsal root ganglion , population , embryogenesis , biochemistry , prostaglandin e , cytosol , embryo , cyclooxygenase , chemistry , biology , medicine , dorsum , microbiology and biotechnology , anatomy , demography , sociology
Newly formed prostaglandins (PGs), which are assumed to act as modulators of afferent sensory messages, were studied in chick dorsal root ganglia (DRG) during development, [1‐ 14 C]Arachidonic acid was converted by DRG homogenates from 1‐week‐old chickens into two major 14 C‐PGs: PGE 2 and PGD 2 . The enzymatic conversion of arachidonic acid was characterized as follows: (a) Boiled preparations were inactivated; (b) synthesis of PGs was inhibited by pretreatment with aspirin or indomethacin and enhanced by esculetin, a protector of cyclooxygenase; and (c) [ 14 C]PGE 2 and [ 14 C]PGD 2 accumulation was a protein dose‐dependent process. Further fractionation of crude homogenates indicated that PG endoperoxide synthetase (EC 1.14.99.1) and PGE 2 synthetase (EC 5.3.99.3) were membrane‐bound enzymes, whereas PGD 2 synthetase (EC 5.3.99.2) was recovered in the cytosol. During development, from embryonic day 10 to day 14 after hatching, PGD 2 synthetase activity remained constant; in contrast, a sharp rise in [ 14 C]PGE 2 synthesis was observed from embryonic day 14 to 18. The time curves of PGD 2 and PGE 2 synthetase specific activity may be related to changes taking place in the cell population of developing DRG. It is therefore suggested that arachidonic acid would be enzymatically converted early into PGD 2 by maturing ganglion cells and then later into PGE 2 by proliferating fibroblasts.