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Effect of Adenosine on 45 Ca 2+ Uptake by Electrically Stimulatad Rat Brain Synapatosomes
Author(s) -
Gongalves M. L.,
Pinto F.,
Ribeiro J. A.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb02079.x
Subject(s) - adenosine , stimulation , calcium , chemistry , inhibitory postsynaptic potential , medicine , biophysics , endocrinology , biochemistry , biology , organic chemistry
The effect of adenosine on 45 Ca 2+ uptake by rat brain synaptosomes stimulated by electrical pulses was investigated. 45 Ca 2+ uptake was voltage dependent. Adenosine (1 μ M ‐1 μ M ) decreased the uptake of 45 Ca 2+ induced by electrical stimulation (amplitude, 20 V; duration, 400 pμs; frequency, 10 pulses/s) in a concentration‐dependent manner. At a concentration of 1 μ M , adenosine almost abolished the 45 Ca 2+ uptake induced by electrical stimulation (92.9 ± 5.3% inhibition), but when the calcium uptake was induced by high‐K + (60 m M ) medium, the effect of adenosine (1 μ M ) was smaller (43.8 ± 5.2% inhibition). The inhibitory effect of 1 μ M adenosine on calcium uptake induced by electrical stimulation was antagonized by 1,3‐dipropyl‐8‐ p ‐sulfophenylxanthine (5 μ M ). The possibility that adenosine interacts with the calcium channels opened by electrical stimulation is discussed.