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Isolation of Monoaminergic Synaptosomes from Rat Brain by Immunomagnetophoresis
Author(s) -
Docherty Maureen,
Bradford H. F.,
Cash C. D.,
Ehret M.,
Maitre M.,
Job Tong H.
Publication year - 1991
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1991.tb02053.x
Subject(s) - monoaminergic , dopaminergic , tyrosine hydroxylase , dopamine , tryptophan hydroxylase , synaptosome , serotonergic , neurotransmitter , chemistry , serotonin , monoamine neurotransmitter , biochemistry , tyrosine 3 monooxygenase , medicine , biology , endocrinology , in vitro , receptor
Monoaminergic synaptosomes have been isolated and purified from rat brain by immunomagnetophoresis. This novel technique uses magnetic beads to which Protein A is bound. Noradrenergic, dopaminergic, and serotonergic synaptosomes (previously cell‐surface labelled with anti‐dopamine‐β‐hydroxylase, anti‐tyrosine hydroxylase, and anti‐tryptophan hydroxylase, respectively) may be isolated in a highly purified state. The synaptosomal subpopulations are recovered in a viable metabolic state and show glucose‐stimulated respiration and Ca 2+ ‐dependent neurotransmitter release. A novel subtype of dopamine‐β‐hydroxylase was found in dopaminergic terminals. No evidence for glutamate core‐lease from monoaminergic synaptosomes was obtained.