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In Vivo Translocation and Down‐Regulation of Protein Kinase C Following Intraventricular Administration of Tetanus Toxin
Author(s) -
Aguilera José,
Yavin Ephraim
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb13319.x
Subject(s) - protein kinase c , cytosol , phosphatidylserine , neurotoxicity , chromosomal translocation , protein kinase a , tetanus , toxin , chemistry , in vivo , biology , intracellular , microbiology and biotechnology , kinase , endocrinology , medicine , enzyme , biochemistry , phospholipid , immunology , membrane , toxicity , vaccination , gene
A single intraventricular injection into adult rats of 100 mouse lethal doses of tetanus toxin (TeTox) produces a marked intracellular redistribution of Ca 2+ /phosphatidylserine (PtdSer)‐dependent protein kinase C (PKC) activity. Changes are particularly pronounced in hypothalamus, hippocampus, and spinal cord structures. Translocation of PKC from the inactive cytosolic compartment to a membrane‐bound active form is followed by a time‐dependent reduction in both total activity and enzyme protein. The down‐regulation of PKC activity in the hypothalamus is accompanied by a marked increase in a Ca 2+ /PtdSer‐independent kinase activity, predominantly in the cytosolic fraction. Our data identify PKC as a possible indirect target for TeTox and suggest that down‐regulation of the enzyme may provide a clue for tetanus neurotoxicity.