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Solubilization and Partial Purification of α‐Amino‐3‐ Hydroxy‐5‐Methyl‐4‐Isoxazolepropionic Acid Binding Sites from Rat Brain
Author(s) -
Hunter C.,
Wheaton K. D.,
Wenthold R. J.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb13290.x
Subject(s) - ampa receptor , chemistry , binding site , potassium thiocyanate , biochemistry , size exclusion chromatography , membrane , amino acid , chromatography , glutamate receptor , receptor , organic chemistry , enzyme
α‐Amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) binding sites were solubilized from rat brain membranes using 1% Triton X‐100 in 0.5 M potassium phosphate buffer containing 20% glycerol. The solubilized binding sites were stable, permitting biochemical and pharmacological characterization as well as partial purification. Pharmacological and binding analyses indicated that the solubilized binding sites were similar to the membrane‐bound sites. Both the solubilized and the membrane‐bound preparations contained high‐ and low‐affinity AMPA binding sites in the presence of potassium thiocyanate. A similar rank order for inhibition of [ 3 H]AMPA binding by several excitatory amino acid analogs was obtained for the soluble and membrane‐bound preparations. [ 3 H]AMPA binding to both soluble and membrane‐bound preparations was increased in the presence of potassium thiocyanate. The solubilized AMPA binding sites migrated as a single peak with gel filtration chromatography, with an M r of 425,000. Beginning with the solubilized preparation, AMPA binding sites were purified 54‐fold with ion‐exchange chromatography and gel filtration. The characterization and purification of these soluble binding sites is potentially useful for the molecular characterization of this putative excitatory amino acid receptor subtype.