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Autopsy Samples of Alzheimer's Cortex Show Increased Peroxidation In Vitro
Author(s) -
Subbarao Kala V.,
Richardson J. Steven,
Ang Lee C.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb08858.x
Subject(s) - lipid peroxidation , cerebral cortex , cortex (anatomy) , cerebellum , thiobarbituric acid , chemistry , glutathione , endocrinology , brain cortex , medicine , alzheimer's disease , autopsy , pathology , biochemistry , biology , antioxidant , neuroscience , enzyme , disease
The formation of thiobarbituric acid‐reactive products was measured as an index of peroxidation by oxygen free radicals in homogenates of frontal cortex and cerebellum from brains taken at autopsy and verified histologically as being Alzheimer's (n = 6) or normal (n = 6). Compared with controls, basal peroxidation is significantly higher in Alzheimer's cortex, and this difference is also evident in the presence of exogenous iron. Peroxidation in cerebellum and levels of total glutathione, RNA, and DNA in cortex and cerebellum do not differ significantly between Alzheimer's brain and controls. Iron‐induced peroxidation in cortex is reduced by the lazaroid U‐74500A, with calculated IC 50 values that are significantly higher in Alzheimer's samples (10 μM ) than in controls (2.5 μM ). These observations suggest that cerebral cortex from Alzheimer's patients differs from controls with respect to in vitro peroxidation.