Molecular Cloning of Microtubule‐Associated Protein 1 (MAP1A) and Microtubule‐Associated Protein 5 (MAP1B): Identification of Distinct Genes and Their Differential Expression in Developing Brain | Zendy

Garner Craig C. | Zendy; Garner Abigail | Zendy; Huber Gerda | Zendy; Kozak Christine | Zendy; Matus Andrew | Zendy

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Molecular Cloning of Microtubule‐Associated Protein 1 (MAP1A) and Microtubule‐Associated Protein 5 (MAP1B): Identification of Distinct Genes and Their Differential Expression in Developing Brain

Author(s) -

Garner Craig C.,

Garner Abigail,

Huber Gerda,

Kozak Christine,

Matus Andrew

Publication year - 1990

Publication title -

journal of neurochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.75

H-Index - 229

eISSN - 1471-4159

pISSN - 0022-3042

DOI - 10.1111/j.1471-4159.1990.tb08832.x

Subject(s) - biology , gene , complementary dna , gene expression , cdna library , northern blot , microbiology and biotechnology , messenger rna , genetics , southern blot , cloning (programming) , transcription (linguistics) , genomic dna , linguistics , philosophy , computer science , programming language

cDNA clones encoding microtubule‐associated proteins 1 (MAP1/MAP1A) and 5 (MAP5/MAP1B) were isolated and have been used to study their structural relationship as well as their regulated expression in developing rat brain. cDNA clones specific for MAP1 hybridized to a single 10‐kb rat brain mRNA, and analysis of genomic DNA by Southern blotting indicated the existence of a single MAP1 gene. A second set of cDNAs specific for MAP5 hybridized to a single 11‐kb mRNA in rat brain and also detected a single gene. By analysis of hybrid mouse‐hamster cell lines, the MAP1 gene was located to mouse chromosome 2, designated Mtap‐1 , and the MAP5 gene to chromosome 13, designated Mtap‐5 MAP1 and MAP5 mRNAs were expressed with different temporal patterns during rat brain development that mirrored the appearance of their protein products, suggesting that expression of these proteins is under transcriptional control. These results taken together demonstrate that although MAP1 and MAP5 have some properties that are similar, they are structurally distinct proteins whose transcription is differently regulated from separate genes.

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