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The Glycine Antagonist 7‐Chlorokynurenic Acid Blocks the Effects of N ‐Methyl‐d‐Aspartate on Agonist‐Stimulated Phosphoinositide Hydrolysis in Guinea‐Pig Brain Slices
Author(s) -
Kendall David A.,
Robinson Jean P.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb05776.x
Subject(s) - nmda receptor , kynurenic acid , glycine , chemistry , guinea pig , agonist , glutamate receptor , glycine receptor , pharmacology , antagonist , biochemistry , amino acid , receptor , biology , endocrinology
Despite having no effect on basal phosphoinositide hydrolysis, N ‐methyl‐d‐aspartate (NMDA) inhibited carbachol‐stimulated accumulation of 3 H‐inositol phosphates and enhanced that due to noradrenaline in guinea‐pig cerebral cortex slices. The glycine antagonist 7‐chlorokynurenic acid inhibited the effects of NMDA and this inhibition was reversed by glycine. The action of 7‐chlorokynurenic acid was not mimicked by strychnine or HA 966 (l‐hydroxy‐3‐aminopyrrolid‐2‐one). l‐Glutamate also inhibited carbacholstimulated accumulation of 3 H‐inositol phosphates, but this inhibition was not blocked by 7‐chlorokynurenic acid. The data are consistent with glycine maintaining tonic control over NMDA receptor activity in guinea‐pig brain.