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Tyrosine Hydroxylase mRNA Concentration in Midbrain Dopaminergic Neurons Is Differentially Regulated by Reserpine
Author(s) -
Pasinetti Giulio M.,
Morgan David G.,
Johnson Steven A.,
Millar Sharon L.,
Finch Caleb E.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04970.x
Subject(s) - reserpine , tyrosine hydroxylase , substantia nigra , ventral tegmental area , midbrain , dopaminergic , locus ceruleus , endocrinology , medicine , in situ hybridization , immunocytochemistry , locus coeruleus , dopamine , biology , pars compacta , messenger rna , neuroscience , central nervous system , biochemistry , gene
Tyrosine hydroxylase (TH)‐mRNA, assayed by in situ hybridization combined with TH immunocytochemistry, showed a selective increase in the ventral tegmental area (A‐10) but not in the substantia nigra (A‐9) midbrain dopaminergic (DAergic) neurons 3 days after reserpine treatment. TH‐mRNA in locus ceruleus noradrenergic (A‐4) neurons was increased by reserpine, as confirmed by RNA blot hybridization. These findings show that TH‐mRNA is differentially regulated in midbrain DAergic neurons in response to reserpine.