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Detection of Cystathionine Ketimine in Bovine Cerebellum
Author(s) -
Ricci G.,
Vesci L.,
Matarese R. M.,
Antonucci A.,
Maggio A.,
Pecci L.,
Cavallini D.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04944.x
Subject(s) - chemistry , chromatography , derivatization , cystathionine beta synthase , gas chromatography , diazomethane , mass spectrometry , sulfur , high performance liquid chromatography , gas chromatography–mass spectrometry , biochemistry , organic chemistry , methionine , amino acid
A new sulfur‐containing cyclic imino acid, cystathionine ketimine, has been detected in bovine cerebellum by gas chromatography, gas chromatography‐mass spectrometry, and high pressure liquid chromatography procedures. Gas chromatography and gas‐mass analyses are based on derivatization of endogenous cystathionine ketimine with diazomethane after a simple enrichment procedure. The high pressure liquid chromatography procedure takes advantage of the selective absorbance at 380 nm of the phenyl isothio‐cyanate‐ketimine interaction product. The concentration of this new sulfur imino acid found in a pool of four bovine cerebella is ∼0.5 nmol/g.