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In Vivo Monitoring of Catecholaminergic Metabolism in the C1 Region of Rat Medulla Oblongata: A Comparative Study by Voltammetry and Intracerebral Microdialysis
Author(s) -
LambásSeñas Laura,
Gillon JeanYves,
Bouilloux JeanPierre,
Seccia Michèle,
Buda Michel,
Renaud Bernard
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04909.x
Subject(s) - microdialysis , chemistry , medulla oblongata , in vivo , tyrosine hydroxylase , catecholaminergic , pargyline , catecholaminergic cell groups , monoamine oxidase , metabolite , catecholamine , pharmacology , biochemistry , endocrinology , central nervous system , biology , enzyme , extracellular , microbiology and biotechnology
In vivo voltammetry or microdialysis was used to monitor catecholaminergic metabolism in the C1 region of the ventrolateral medulla oblongata of anesthetized rats. In vivo voltammetry allowed the recording of a catechol oxidation current (CA.OC) peak in this region. This CA.OC was suppressed after inhibition of monoamine oxidase by pargyline or after inhibition of tyrosine hydroxylase by α‐methyl‐ p ‐tyrosine and was markedly increased after blockade of dopamine‐ β ‐hydroxylase by FLA 63. Similar results were found when intracerebral microdialysis coupled with HPLC and electrochemical detection was used to measure the concentration of 3,4‐dihydroxyphenylacetic acid (DOPAC) in the dialysates obtained from the C1 region: The changes in CA.OC and DOPAC concentration in the dialysates exhibited very similar kinetic characteristics in the three pharmacological experiments. These results support the involvement of DOPAC as a major component of the electrochemical signal recorded by voltammetry in the C1 group of adrenergic neurons.