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Developmental Expression of G o in Neuronal Cultures from Rat Mesencephalon and Hypothalamus
Author(s) -
Granneman James G.,
Kapatos Gregory
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04903.x
Subject(s) - hypothalamus , midbrain , neuron , biology , endocrinology , population , medicine , in vitro , microbiology and biotechnology , central nervous system , neuroscience , biochemistry , environmental health
The developmental expression of the α ‐subunit of G o was examined in neuronal cultures derived from rat mesencephalon (MES) and hypothalamus (HYP). These cultures were essentially free of contaminating glia and were maintained as a stable population for periods up to 3 weeks. Immunoblotting utilizing specific antisera against G o indicated that in neurons from both brain regions, membrane concentrations of G o increased dramatically during the first 2 weeks in vitro. Thereafter, increases in the amount of G o per neuron kept pace with increasing process (axons and dendrites) formation. Multiple forms of immunoreactive G o were detected in MES and HYP neurons, and the proportions of these forms changed between 4 and 14 days in culture. Finally, increasing neuron density significantly increased membrane levels of G o in MES but not HYP cultures.