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1,2‐Diacylglycerol Content and Its Arachidonyl‐Containing Molecular Species Are Reduced in Sciatic Nerve from Streptozotocin‐induced Diabetic Rats
Author(s) -
Zhu Xi,
Eichberg Joseph
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04604.x
Subject(s) - diacylglycerol kinase , sciatic nerve , chemistry , endocrinology , medicine , streptozotocin , diabetes mellitus , protein kinase c , biochemistry , biology , kinase
The content of 1,2‐diacylglycerol (DAG) was determined in sciatic nerves from normal and streptozotocin‐induced diabetic rats. In nerves frozen in situ, DAG content was reduced 22% in the proximal region and 77% in the distal region of diabetic nerve, principally because of the loss of associated fat. DAG levels in freshly dissected and desheathed diabetic nerve were decreased from 23 to 30% as compared with normal nerve. Determination of DAG molecular species distribution in desheathed normal nerve indicated that 18:0/20:4 accounted for 34%, 16:0/18:1 for 17%, and several other polyunsaturated fatty acid‐containing species for 17% of the total. In diabetic nerve, the quantity of the 18:0/20:4 DAG species was reduced by 37%, and this drop was 62% of the reduction in all molecular species. The content of the minor species, 16:0/20:4 DAG, was decreased by 48%. Our results suggest that nerve DAG arises in large part from phosphoinositide degradation. Moreover, these results provide support for the hypothesis that reduced Na + ,K + ‐ATPase activity in diabetic nerve is a consequence of decreased phosphoinositide turnover, which thereby generates insufficient DAG to maintain a protein kinase C‐mediated step necessary for activation of Na + ,K + ‐ATPase.