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Protein Kinase C Activity in Rat Brain Cortex
Author(s) -
Crumrine R. Christian,
LaManna Joseph C.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04566.x
Subject(s) - protein kinase c , cerebral cortex , cortex (anatomy) , specific activity , chemistry , brain tissue , enzyme , protein kinase a , enzyme assay , biochemistry , biology , endocrinology , medicine , anatomy , neuroscience
The procedure used to obtain cerebral tissue for analysis of protein kinase C (PKC) activity may affect the subcellular distribution of the enzyme. We compared different methods of tissue preparation and found that the proportion of PKC activity associated with the paniculate fraction of the cerebral cortex was only 30% when the brain was frozen in situ while the animal was on life support or after decapitation followed by delayed freezing. Other methods of obtaining cerebral tissue resulted in 49–56% of the PKC activity in the particulate fraction. Freezing per se had no apparent effect on the activity or subcellular distribution of PKC. In addition, whenever the particulate PKC activity was high (>48%), there was also a significant increase in the proportion of particulate protein (from 51 to ∼63%, p > 0.05).