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Imaging the Dopamine Uptake Site with Ex Vivo [ 18 F]GBR 13119 Binding Autoradiography in Rat Brain
Author(s) -
Ciliax Brian J.,
Kilbourn Michael R.,
Haka Michael S.,
Penney John B.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04178.x
Subject(s) - dopamine , olfactory tubercle , nucleus accumbens , striatum , substantia nigra , ventral tegmental area , chemistry , dopamine uptake inhibitors , binding site , dopamine receptor d1 , medicine , neuroscience , dopamine receptor , endocrinology , pharmacology , biology , biochemistry , dopaminergic
We studied the binding of [ 18 F]GBR 13119 {1‐[[(4‐[ 18 F]fluorophenyl) (phenyl)methoxy]ethyl]‐4‐(3‐phenylpropyl)piperazine} to rat brain with autoradiography after intravenous injection. The rank order of binding was dorsal striatum > nucleus accumbens = olfactory tubercle > sub‐stantia nigra = ventral tegmental area > other areas. Binding was blocked by prior injection of dopamine uptake blockers but not by injection of dopamine receptor antagonists or drugs that bind to the dialkylpiperazine site. Unilateral 6‐hydroxy dopamine lesions of dopamine neurons caused a marked decrease in striatal and nigral binding on the side of the lesion. We conclude that intravenous injection of [ 18 F]GBR 13119 provides a useful marker of presynaptic dopamine uptake sites.