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Autoradiographic Localization of Angiotensin II Receptor Binding Sites on Noradrenergic Neurons of the Locus Coeruleus of the Rat
Author(s) -
Rowe Brian P.,
Kalivas Peter W.,
Speth Robert C.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb04167.x
Subject(s) - locus coeruleus , ibotenic acid , norepinephrine , endocrinology , lesion , medicine , neurotoxin , catecholamine , chemistry , microinjection , angiotensin ii , brainstem , receptor , neuroscience , central nervous system , biology , dopamine , pathology
The locus coeruleus (LC) of the rat was lesioned by microinjection of selective neurotoxins into the brainstem. 6‐Hydroxydopamine (6‐OHDA), 3 μg/μl, given unilaterally at two sites 0.6 mm apart on the rostro‐caudal axis of the LC, was used to lesion catecholamine‐containing neuronal elements. Ibotenic acid, 2.5 μg/0.5 μl, administered similarly was used to lesion nerve cell bodies. Two weeks after administration of the neurotoxin, lesion efficacy was determined based on the norepinephrine content of the cerebral cortex ipsi‐ and contralateral to the lesion. 6‐OHDA lesions of the LC caused a 46% reduction in ipsilateral cortical norepinephrine and a 60% reduction in specific 125 I‐[Sar 1 , Ile 8 ]‐angiotensin II ( 125 I‐SIAII) binding in the LC. Ibotenic acid lesions of the LC caused a 73% reduction in ipsilateral cortical norepinephrine and a 81% reduction in specific 125 I‐SIAII binding in the LC. These results indicate that All receptor binding sites in the LC are localized on noradrenergic nerve cell bodies or their dendritic and axonal ramifications within the LC.