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Determination of Gangliosides in Six Human Primary Medulloblastomas
Author(s) -
Gottfries Johan,
Fredman Pam,
Mansson JanEric,
Collins V. Peter,
Hoist Hans,
Armstrong Dawna D.,
Percy Alan K.,
Wikstrand Carol J.,
Bigner Darell D.,
Svennerholm Lars
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb03142.x
Subject(s) - ganglioside , sialic acid , sialidase , monoclonal antibody , biochemistry , staining , chemistry , hydrolysis , biology , antibody , microbiology and biotechnology , neuraminidase , immunology , enzyme , genetics
The ganglioside composition of six human medulloblastomas was analyzed. The characterization was performed by thin‐layer chromatography, sialidase hydrolysis, and immunological staining with a panel of characterized antiganglioside monoclonal antibodies. The total ganglioside content ranged from 60 to 1,130 nmol of ganglioside sialic acid/g wet weight. Neuronal gangliosides (gangliotetraose series) were found in varying amounts in all medulloblastomas. Gangliosides of the neolactotetraose series (3′‐LM 1 and LD1) were present in all specimens, and the lactotetraose series ganglioside 3′‐isoLM1 was found in all cases showing astro‐cytic differentiation. This supports our previous findings that 3′‐isoLM1 is a marker for proliferating astroglial cells.