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Effects of Transforming Growth Factor β 1 on Astroglial Cells in Culture
Author(s) -
ToruDelbauffe D.,
BaghdassarianChalaye D.,
Gavaret J. M.,
Courtin F.,
Pomerance M.,
Pierre M.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb02357.x
Subject(s) - transforming growth factor , astrocyte , dna synthesis , glutamine synthetase , cell culture , growth factor , endocrinology , glutamine , medicine , regulator , chemically defined medium , biology , primary culture , fibroblast growth factor , transforming growth factor beta , chemistry , microbiology and biotechnology , biochemistry , dna , in vitro , amino acid , genetics , gene , receptor , central nervous system
The effects of transforming growth factor β 1 (TGF β 1) on DNA synthesis and functional differentiation of astroglial cells cultured in serum‐free medium were investigated. TGF β 1 diminished and delayed the peak of DNA synthesis induced by serum. TGF β 1‐treated cells were larger than control cells. This factor delayed the appearance of process‐bearing cells induced by acidic fibroblast growth factor treatment and also affected the astrocyte‐specific enzyme glutamine synthetase (GS), whose accumulation is under hydrocortisone (HC) control. TGF β 1 inhibited the induction of GS activity by HC in a dose‐ and time‐dependent manner. Moreover, pretreatment with TGF β 1 for 4 h maintained the inhibition of GS activity for ˜16 h after removal of this factor from culture medium. These results suggest that TGF β 1 may be an important regulator of astrocyte growth and differentiation.