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Release of Calcitonin Gene‐Related Peptide‐Like Immunoreactive Substance from Neuromuscular Junction by Nerve Excitation and Its Action on Striated Muscle
Author(s) -
Uchida Shuji,
Yamamoto Hiromasa,
Iio Sadaharu,
Matsumoto Naoko,
Wang XiaoBing,
Yonehara Norifumi,
Imai Yasuo,
Inoki Reizo,
Yoshida Hiroshi
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb02349.x
Subject(s) - calcitonin gene related peptide , phrenic nerve , calcitonin , stimulation , medicine , endocrinology , contraction (grammar) , neuromuscular junction , substance p , chemistry , muscle contraction , neuropeptide , neuroscience , biology , receptor , respiratory system
In a rat phrenic nerve‐hemidiaphragm preparation, calcitonin gene‐related peptide (CGRP) increased the twitch contraction induced by nerve or transmural stimulation dose dependently. Either electrical or high K + stimulation of the phrenic nerve caused release of a CGRP‐like immunoreactive substance (CGRP‐LIS) in a Ca 2+ ‐dependent manner. Electrical stimulation of the phrenic nerve also increased the cyclic AMP content in diaphragm. This increase was not observed in Ca 2+ ‐free medium and was blocked by antiserum against CGRP. These results indicate that excitation of the motor nerve causes release of CGRP‐LIS at nerve terminals and that the released CGRP‐LIS increases the cyclic AMP content of skeletal muscles and potentiates twitch contraction.