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Chicken Growth‐Associated Protein GAP‐43 Is Tightly Bound to the Actin‐Rich Neuronal Membrane Skeleton
Author(s) -
Moss D. J.,
Fernyhough P.,
Chapman K.,
Baizer L.,
Bray D.,
Allsopp T.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb02312.x
Subject(s) - biology , membrane glycoproteins , glycoprotein , antiserum , biochemistry , actin , phosphorylation , microbiology and biotechnology , membrane protein , complementary dna , protein kinase a , membrane , antigen , gene , genetics
We have identified the chicken equivalent of growth‐associated protein GAP‐43 in a detergent‐resistant membrane skeleton from cultures of chick neurones and embryonic chick brain. Antisera to the membrane skeleton protein, the 3D5 antigen, precipitate the translation product of chick GAP‐43 cDNA, and the 3D5 antigen is also detected by antisera against synthetic peptides from the known amino acid sequence of rat GAP‐43. The chick protein and the rat GAP‐43 are biochemically similar proteins that both serve as major targets of phosphorylation by endogenous protein kinase C. The detergent‐resistant complex in which GAP‐43 is found also contains actin ˜5% of the total protein) and a neurone‐specific cell surface glycoprotein. We suggest that the membrane skeleton of neurones may be a primary site of action of GAP‐43.