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Selective Increase in S‐100β Protein by Aging in Rat Cerebral Cortex
Author(s) -
Kato Kanefusa,
Suzuki Fujiko,
Morishita Rika,
Asano Tomiko,
Sato Tsuneko
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01958.x
Subject(s) - enolase , cerebellum , cerebral cortex , brainstem , central nervous system , cortex (anatomy) , endocrinology , biology , medicine , biochemistry , neuroscience , immunohistochemistry
Changes in the cocentrations of nervous tissuerelated proteins and their isoproteins, such as S‐100 proteins (S‐100α and S‐100β), enolase isozymes (α‐enolase and γ‐enolase), and GTP‐binding proteins (G oα , G i2α , and β‐subunits), were determined in the CNS of male rats of various ages (from 2 to 30 months old) by means of enzyme immunoassay. The weights of brains and the concentrations of soluble proteins in the cerebral cortex, cerebellum, and brainstem were constant during the observation period. The concentration of S‐100β protein, which is predominantly localized in glial cells, increased gradually in the cerebral cortex with age; levels in the 25‐month‐old rats increased to approximately 150% of the levels in the young (2‐month‐old) rats. However, the S‐100β concentrations in the cerebellum and brainstem were relatively constant, showing similar values in rats 2–30 months old. Levels of other proteins, including both neuronal (γ‐enolase and G oα ) and glial (α‐enolase and S‐100α) marker proteins, did not change significantly with age in the cerebral cortex, cerebellum, and brainstem. These results suggest that there is a close relation between the age‐dependent changes of the CNS function and S‐100β protein levels in the cerebral cortex.