Premium
Production and Characterization of Monoclonal Antibodies to the Major Peripheral Myelin Glycoprotein P 0
Author(s) -
Bollensen Edgar,
Scheidt Peter,
Schachner Melitta
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01936.x
Subject(s) - monoclonal antibody , glycoprotein , antibody , myelin , peripheral nervous system , epitope , central nervous system , microbiology and biotechnology , blot , subclass , biology , myelin associated glycoprotein , nervous system , immunology , biochemistry , endocrinology , neuroscience , gene
Several monoclonal antibodies were generated against the major glycoprotein P 0 of human peripheral nervous system myelin. Antibodies were selected for their reactivity with P 0 in Western blots. The antibodies were of the immunoglobulin G subclass and reacted with the glycopeptidase F‐treated P 0 , indicating that the reactive epitope resides in the protein backbone. In fresh frozen and paraffin‐embedded sections of central and peripheral nervous system of rat and human, P 0 antibody 592 reacted with myelin sheaths of peripheral, but not central, nervous system.