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A New Enzymatic Cycling Technique for Glutamate Determination in Brain Microdialysates
Author(s) -
Mitani Akira,
Kubo Hirohiko,
Iga Kouzo,
Imon Hitoshi,
Kadoya Fumito,
Kataoka Kiyoshi
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01930.x
Subject(s) - cycling , glutamate receptor , enzyme , chemistry , biochemistry , receptor , archaeology , history
A quantitative analysis of glutamate in brain dialysate was made by using an enzymatic cycling technique. This method made it possible to measure the concentration of glutamate in dialysate collected at 30‐s intervals. Dialysates were collected from Mongolian gerbil hippocampus before, during, and after two 90‐s ischemic insults at an interval of 5 min. An extracellular increase in levels of glutamate was already observed in samples collected during a 30–60 s period after the onset of each ischemia, and the levels of glutamate were maximal at the end of each period of ischemia (approximately a fourfold increase). The increased levels of glutamate rapidly returned almost to preischemic levels by 30 s of recirculation. This method will provide more precise information about temporal changes in the extracellular glutamate concentration in the brain during ischemia.