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Expression of a Human Cocaine‐Sensitive Dopamine Transporter in Xenopus laevis Oocytes
Author(s) -
Ban Michael J.,
Xue ChangHong,
Shibata Kazuhiko,
Dragovic Ljubisa J.,
Kapatos Gregory
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01929.x
Subject(s) - dopamine transporter , dopamine , xenopus , oocyte , dopamine plasma membrane transport proteins , substantia nigra , endocrinology , medicine , chemistry , biology , microbiology and biotechnology , dopaminergic , biochemistry , embryo , gene
The human dopamine transporter was expressed in Xenopus laevis oocytes following injection of mRNA isolated from human brain substantia nigra. The specific accumulation of [ 3 H]dopamine into these oocytes was time and Na + dependent. Furthermore, [ 3 H]dopamine accumulation was prevented by coincubation of oocytes with dopamine (100 μM ) or with the dopamine uptake inhibitors GBR 12909 (1 μM ) or cocaine (3 μM ). In contrast, oocyte injection of mRNA isolated from human globus pallidus, an area devoid of dopamine neuron perikarya, did not elicit expression of the dopamine transporter. Oocyte expression of the human dopamine transporter can be used for the further characterization and cloning of this low‐abundance membrane protein.