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Estimation of Free Dopamine in the Cytoplasm of the Giant Dopamine Cell of Planorbis corneus by Voltammetry and Capillary Electrophoresis
Author(s) -
Chien Jennifer B.,
Wallingford Ross A.,
Ewing Andrew G.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01918.x
Subject(s) - dopamine , cytoplasm , capillary electrophoresis , chemistry , microscale chemistry , extracellular , biophysics , biology , chromatography , biochemistry , neuroscience , mathematics education , mathematics
Voltammetric electrodes having a tip diameter of 2–12 μ and microscale capillary zone electrophoresis have been used for dynamic and static monitoring of dopamine in the somal cytoplasm of the giant dopamine neuron of Planorbis corneus . Intracellular dopamine levels can be altered by extracellular application of dopamine or ethanol. The latter experiment provides a means to estimate total stores of endogenous dopamine, and the evidence presented suggests that at least 98% of these stores are bound and not directly accessible to the cytoplasm.