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An Improved HPLC‐Electrochemical Detection Method for Measuring Brain Levels of 5‐ S ‐Cysteinyldopamine, 5‐ S ‐Cysteinyl‐3,4‐Dihydroxyphenylalanine, and 5‐ S ‐Cysteinyl‐3,4‐Dihydroxyphenylacetic Acid
Author(s) -
Fornstedt Bodil,
Bergh Ingrid,
Rosengren Evald,
Carlsson Arvid
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01910.x
Subject(s) - dihydroxyphenylalanine , neuroscience , chemistry , high performance liquid chromatography , psychology , chromatography , dopamine
Abstract: Brain levels of the 5‐ S ‐cysteinyl adducts of 3,4‐dihydroxyphenylalanine (DOPA), 3,4‐dihydroxyphenylacetic acid (DOPAC), and dopamine were determined in several mammalian species. The low levels of the compounds and the risk of artifacts during sample preparation necessitated rather profound modifications of the assaying method. The refined method has made it possible to present more accurate data than those previously reported from this laboratory. The occurrence of low levels of the 5‐ S ‐cysteinyl adducts in dopamine‐rich brain areas, but not in cerebellum, is indirect evidence of in vivo autoxidation of DOPA, DOPAC, and dopamine. The products generated during catechol autoxidation, including quinones and reduced forms of oxygen, are known to be potentially cytotoxic.