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Retinoic Acid Rapidly Decreases Phosphatidylinositol Turnover During Neuroblastoma Cell Differentiation
Author(s) -
Ponzoni M.,
Lanciotti M.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01905.x
Subject(s) - retinoic acid , phosphatidylinositol , neuroblastoma , chemistry , microbiology and biotechnology , endocrinology , medicine , biology , biochemistry , signal transduction , cell culture , genetics , gene
Phosphatidylinositol (PI) turnover has recently been implicated in the regulation of cell proliferation and transformation. We have investigated its role in differentiation using LAN‐1 cells, a human neuroblastoma cell line that can be induced to differentiate along the neuronal pathway by retinoic acid (RA). We have found that treatment of LAN‐1 cells with RA is followed by a rapid decrease of inositol phospholipid metabolism, using myo ‐[1,2‐ 3 H]inositol or [1(3)‐ 3 H]glycerol. No changes were observed in both [ 3 H]inositol and [ 3 H]glycerol uptake within 24 h of RA treatment. Decreased incorporation of the metabolic precursor into PI 4‐monophosphate and PI 4,5‐bisphosphate occurred within 1 h of RA treatment. No changes were seen in the specific radioactivity of the precursor pools up to 1 h of treatment with RA. Analysis of labeled PI metabolites from prelabeled cells indicated a rapid decrease of inositol 1,4,5‐trisphosphate and 1,2‐diacylglycerol content within 1 min of induction of LAN‐1 cell differentiation. These findings constitute the earliest reported events in neuroblastoma cell differentiation.