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Cyclic GMP Regulates Nicotine‐Induced Secretion from Cultured Bovine Adrenal Chromaffin Cells: Effects of 8–Bromo–Cyclic GMP, Atrial Natriuretic Peptide, and Nitroprusside (Nitric Oxide)
Author(s) -
O'Sullivan Antony J.,
Burgoyne Robert D.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01238.x
Subject(s) - atrial natriuretic peptide , medicine , endocrinology , nitric oxide , nicotine , bradykinin , secretion , chemistry , adrenal medulla , chromaffin cell , angiotensin ii , biology , catecholamine , receptor
Methacholine, atrial natriuretic peptide (ANP), nitroprusside (nitric oxide), angiotensin II. and bradykinin raised cyclic. GMP (cGMP) levels in cultured bovine adrenal chromaffin cells. The role of cGMP in secretion from chromaffin cells was examined using 8‐bromo‐cGMP. This analogue had no effect on basal secretion or secretion due to angiotensin II, bradykinin, or a high K + level but potentiated secretion due to low doses of nicotine. At supramaximal doses of nicotine, 8‐bromo‐cGMP inhibited secretion. These effects of 8‐bromo‐cGMP were not due to changes in the nicotine‐induced rise in cytosolic calcium concentration. A potentiation of secretion due to low doses of nicotine was also found following simultaneous addition of ANP or nitroprusside, a result suggesting that ANP and nitric oxide (endothelium‐derived relaxing factor) could be important regulators of secretion from adrenal chromaffin cells.