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Characterization of the Quisqualate Receptor Linked to Phosphoinositide Hydrolysis in Neurocortical Cultures
Author(s) -
Patel Jitendra,
Moore W. Craig,
Thompson Carolann,
Keith Richard A.,
Salama Andre I.
Publication year - 1990
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1990.tb01192.x
Subject(s) - kainate receptor , quisqualic acid , nmda receptor , biochemistry , ampa receptor , excitatory postsynaptic potential , inositol phosphate , amino acid , glutamate receptor , biology , propionate , excitatory amino acid antagonists , chemistry , inositol , receptor
Activation of phosphoinositide metabolism is an early event in signal transduction for a number of neurotransmitters and hormones. In primary cultures of rat neurocortical cells, various excitatory amino acids stimulate inositol phosphate production with a rank order of potency of quisqualate > ibotenate > glutamate > kainate, N ‐methyl‐ d ‐aspartate > α‐amino‐3‐hydroxyl‐5‐methyl‐4‐isoxazole propionate. This response to excitatory amino acids was insensitive to a variety of excitatory amino acid antagonists including 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione, 3–3(2‐carboxypiperazine‐4‐yl)propyl‐l‐phosphonate, and 2‐amino‐4‐phosphonobutyrate. The individual responses of quisqualate‐, ibotenate‐, and kainate‐stimulated inositol phosphate production were not additive. These results suggest that phosphoinositide metabolism activated by excitatory amino acids is mediated by a unique quisqualate‐preferring receptor that is not antagonized by known N ‐methyl‐ d ‐aspartate and non‐ N ‐methyl‐ d ‐aspartate antagonists, and is relatively insensitive to α‐amino‐3‐hydroxyl‐5‐methyl‐4‐isoxazole propionate.