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tele ‐Methylhistamine Levels and Histamine Turnover in Nuclei of the Rat Hypothalamus and Amygdala
Author(s) -
Itoh Yoshinori,
Oishi Ryozo,
Nishibori Masahiro,
Saeki Kiyomi
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb11781.x
Subject(s) - histamine , hypothalamus , amygdala , medicine , endocrinology , pargyline , chemistry , nucleus , biology , neuroscience , serotonin , receptor
An HPLC method using fluorescence detection for the determination of tele ‐methylhistamine! (t‐MH) was improved to a sensitivity level which enabled the detection of 0.05 pmol of tissue t‐MH. The t‐MH contents and the histamine turnover rates in various nuclei of the rat hypothalamus and amygdala were subsequently measured. The histamine turnover rates were estimated from pargyline‐induced t‐MH accumulation. Both the t‐MH levels and the histamine turnover rates were shown to be relatively high in the nuclei dorsomedialis and premammillaris ventralis of the hypothalamus, and also in the nucleus medialis of the amygdala. The steady‐state t‐MH levels in various nuclei of the hypothalamus and amygdala correlated well with the histamine turnover rates in these nuclei.