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Direct Transfer into Nitrocellulose and Quantitative Radioautographic Anatomical Determination of Brain Tyrosine Hydroxylase Protein Concentration
Author(s) -
Weissmann Dinah,
Labatut René,
Richard Françoise,
Rousset Colette,
Pujol JeanFrançois
Publication year - 1989
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1989.tb11775.x
Subject(s) - locus ceruleus , reserpine , tyrosine hydroxylase , substantia nigra , chemistry , nitrocellulose , quantitative analysis (chemistry) , locus coeruleus , endocrinology , medicine , biochemistry , biology , dopamine , chromatography , enzyme , central nervous system , dopaminergic , membrane
An improved quantitative immunochemical determination of brain tyrosine hydroxylase (TH) concentra‐tions was designed using direct transfer into nitrocellulose from 20‐μm thick brain sections, followed by immunodetection and quantitative radioautography in three reference brain structures (locus ceruleus, substantia nigra, and ventral tegmental area). Results obtained by this methodology were similar to those obtained after extraction and Western blotting of the TH protein in control and reserpine‐treated animals. Moreover, this methodology allows the combination of high sensitivity and high anatomical resolution in the study of the distribution of pharmacological effects. The locus ceruleus exhibited a significant posteroanterior distribution of TH protein concentration in control and reserpine‐treated animals.